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Conduritol B epoxide

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Purity:100%

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COA HNMR HPLC

Conduritol B epoxide

Catalog No. TQ0300Cas No. 6090-95-5

Conduritol B epoxide is an irreversible inhibitor of β-glucosidase (GCase).

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Pack Size	Price	Availability
5 mg	$41	In Stock
10 mg	$80	In Stock
25 mg	$105	In Stock
50 mg	$174	In Stock
100 mg	$259	In Stock
200 mg	$388	In Stock

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Product Introduction

Bioactivity

Description	Conduritol B epoxide is an irreversible inhibitor of β-glucosidase (GCase).
In vitro	Treating N2a and Conduritol B epoxide-N2a cells with G6 gave GC reductions to 7% and 26% of untreated levels, respectively. G6 treated Conduritol B epoxide-N2a also has significantly decreased GS. Conduritol B epoxide-N2a cells have higher calcium levels than in N2a cells without caffeine at baseline. Conduritol B epoxide-N2a cells show a significant increase in cytosolic calcium levels compared to N2a cells. Conduritol B epoxide-N2a cells show a significant reduction in OCR as evidenced by approximately 50% in all the parameters, including the rate of ATP production, basal respiration, and maximal respiration, compared to N2a cells. In dantrolene-treated Conduritol B epoxide-N2a cells, levels of Ryr3 are increased to 76% of the WT level [1].
In vivo	Treatment with Conduritol B epoxide (100 mg/kg/day, i.p.) administered to 4L, 9H, 9V, and WT mice from postnatal days 5 to 11 does not result in the formation of α-synuclein aggregates. However, prolonged daily administration of this compound to 4L mice (at the same dosage starting from postnatal day 15 for either 24 or 36 doses) causes hind limb paralysis and leads to minimal α-synuclein accumulation in specific areas of the brain, including the olfactory bulb, brainstem, and periventricular region near the dorsal third ventricle (D3V) [2].
Kinase Assay	Cells are homogenized in 1% sodium taurocholate/1% Triton X-100. GCase activity is determined fluorometrically using 4MU-Glucose as the substrate in 0.25% sodium taurocholate, 0.25% Triton X-100 and 0.1M citric-phosphate buffer (pH 5.6). Brain tissues are homogenized in 1X PBS and incubated in 5 μM brain phosphatidylserine and 0.1M citric-phosphate buffer (pH 5.6) for GCase activity assay using 4MU-Glucose as substrate. Protein concentrations are determined by BCA assay using BSA as standard [1].
Animal Research	Gba1 point mutated 4L, 9H and 9V mice or WT mice are intraperitoneally injected with 100 mg/kg/day of Conduritol B epoxide. In short-term experiments, daily injections are initiated at postnatal day 5 and continued for 6 daily doses. The mice are sacrificed on day 12 or 2 months after the last injection. In long-term experiments, 4L mice are injected daily beginning at postnatal day 15 for 24 or 36 daily doses and sacrificed the day after the last injection. Mice are perfused with PBS and organs are harvested for enzyme activity, lipid, and histological analyses [2].

Chemical Properties

Molecular Weight	162.14
Formula	C6H10O5
Cas No.	6090-95-5

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 70 mg/mL (431.73 mM)

H2O: ≥20 mg/mL (123.35 mM)

Solution Preparation Table

DMSO/H2O

	1mg	5mg	10mg	50mg
1 mM	6.1675 mL	30.8375 mL	61.6751 mL	308.3755 mL
5 mM	1.2335 mL	6.1675 mL	12.3350 mL	61.6751 mL
10 mM	0.6168 mL	3.0838 mL	6.1675 mL	30.8375 mL
20 mM	0.3084 mL	1.5419 mL	3.0838 mL	15.4188 mL
50 mM	0.1234 mL	0.6168 mL	1.2335 mL	6.1675 mL
100 mM	0.0617 mL	0.3084 mL	0.6168 mL	3.0838 mL

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Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

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